Question 1

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Accepted Answer

A) True 
 B)False

Question 2

Discuss some of the impacts that tools and techniques of recombinant DNA technology have had on medicine.

Accepted Answer

Human gene libraries and DNA sequencing ...

Question 3

Which of the following procedures would be used to introduce DNA into a single mouse cell?

Accepted Answer

A)   Southern blotting
B)   electroporation
C)   gene gun
D)   microinjection
E)   protoplast fusionF) B) and D)
G) C) and D)

Question 4

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

Accepted Answer

The answer of Nucleic acid molecules used to deliver new...

Question 5

Double strand DNA fragments are separated by size using

Accepted Answer

A)   restriction analysis.
B)   FISH.
C)   PCR.
D)   northern blotting.
E)   gel electrophoresis.F) A) and B)
G) A) and C)

Question 6

If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?

Accepted Answer

A)   5000 base pairs
B)   750 base pairs
C)   1000 base pairs
D)   250 base pairs
E)   2500 base pairsF) C) and E)
G) C) and D)

Question 7

Compare and contrast the two major categories of restriction enzymes.

Accepted Answer

All restriction enzymes recognize specif...

Question 8

Replacing a patient's defective gene with a fragment containing a functional gene is

Accepted Answer

A)   gene therapy.
B)   genomics.
C)   gene cloning.
D)   gene mapping.
E)   gene selection.F) None of the above
G) A) and B)

Question 9

Which of the following restriction enzyme sites would produce blunt-ended fragments? (The arrow represents the cutting site of the enzyme.)

Accepted Answer

A)   C↓CGG
B)   G↓GATCC
C)   G↓AATTC
D)   CCC↓GGG
E)   A↓AGCTTF) C) and E)
G) A) and D)

Question 10

Restriction enzymes are useful only on synthetic DNAs.

Accepted Answer

A) True 
 B)False

Question 11

Sticky-end fragments generated by EcoRI will hydrogen bond to any other sticky-end sequence.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following is NOT a goal of recombinant DNA technology?

Accepted Answer

A)   combining genetic material from more than one organism to produce new useful organisms
B)   making it possible to clone humans
C)   eliminating undesirable traits from livestock or crops
D)   being able to remove or correct damaging traits in humans
E)   creating organisms capable of producing useful productsF) C) and D)
G) D) and E)

Question 13

Synthetic nucleic acids are useful as

Accepted Answer

A)   DNA probes.
B)   primers for PCR.
C)   antisense RNAs.
D)   DNA probes and antisense RNAs.
E)   DNA probes, primers, and antisense RNAs.F) A) and B)
G) A) and C)

Question 14

Reverse transcriptase synthesizes a DNA molecule from an RNA template.

Accepted Answer

A) True 
 B)False

Question 15

Outline a procedure for producing a useful new recombinant product.

Accepted Answer

Many variations are possible.The answer ...

Question 16

Single-strand DNA molecules are fixed to a glass slide or nylon membrane for the production of

Accepted Answer

A)   microarrays.
B)   DNA clones.
C)   real time PCR probes.
D)   FISH assays.
E)   templates for Sanger sequencing.F) A) and B)
G) A) and E)

Question 17

Which of the following is generally NOT considered an ethical issue regarding recombinant DNA technology?

Accepted Answer

A)   the modification of crop plants
B)   screening of humans for genes that predispose them to disease
C)   unforeseen impact on the environment
D)   the modification of animals to produce pharmaceuticals for humans
E)   answering basic research questionsF) B) and D)
G) D) and E)

Question 18

Small single strand DNA molecules with a fluorescent molecule attached that are used to identify a clone containing a specific gene fragment is called a

Accepted Answer

A)   primer.
B)   probe.
C)   plasmid.
D)   promoter.
E)   peptide.F) All of the above
G) C) and E)

Question 19

Transgenic organisms

Accepted Answer

A)   contain cells from other organisms.
B)   contain genetically engineered microbes.
C)   are the same as clones.
D)   contain genes from other organisms.
E)   have genomes that have been sequenced completely.F) A) and B)
G) D) and E)

Question 20

Which of the following devices is used for PCR?

Accepted Answer

A)   an electrophoresis chamber
B)   a gene gun
C)   a DNA sequencer
D)   a thermocycler
E)   a nucleic acid synthesis machineF) C) and E)
G) B) and D)

Exam 8: Recombinant DNA Technology

Outline a procedure for producing a useful new recombinant product.

Correct AnswerverifiedMany variations are possible.The answer ...View AnswerShow Answer

Which of the following is generally NOT considered an ethical issue regarding recombinant DNA technology?

Correct AnswerverifiedShow Answer

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Correct AnswerverifiedShow Answer

Transgenic organisms

Correct AnswerverifiedShow Answer

Single-strand DNA molecules are fixed to a glass slide or nylon membrane for the production of

Correct AnswerverifiedShow Answer

Which of the following devices is used for PCR?

Correct AnswerverifiedShow Answer

Discuss some of the impacts that tools and techniques of recombinant DNA technology have had on medicine.

Correct AnswerverifiedHuman gene libraries and DNA sequencing ...View AnswerShow Answer

Compare and contrast the two major categories of restriction enzymes.

Correct AnswerverifiedAll restriction enzymes recognize specif...View AnswerShow Answer

Double strand DNA fragments are separated by size using

Correct AnswerverifiedShow Answer

Which of the following is NOT a goal of recombinant DNA technology?

Correct AnswerverifiedShow Answer

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

Correct AnswerverifiedShow Answer

Which of the following procedures would be used to introduce DNA into a single mouse cell?

Correct AnswerverifiedShow Answer

Synthetic nucleic acids are useful as

Correct AnswerverifiedShow Answer

Small single strand DNA molecules with a fluorescent molecule attached that are used to identify a clone containing a specific gene fragment is called a

Correct AnswerverifiedShow Answer

Restriction enzymes are useful only on synthetic DNAs.

Correct AnswerverifiedShow Answer

Which of the following restriction enzyme sites would produce blunt-ended fragments? (The arrow represents the cutting site of the enzyme.)

Correct AnswerverifiedShow Answer

Replacing a patient's defective gene with a fragment containing a functional gene is

Correct AnswerverifiedShow Answer

Reverse transcriptase synthesizes a DNA molecule from an RNA template.

Correct AnswerverifiedShow Answer

Sticky-end fragments generated by EcoRI will hydrogen bond to any other sticky-end sequence.

Correct AnswerverifiedShow Answer

If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?

Correct AnswerverifiedShow Answer

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Many variations are possible.The answer ...
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Human gene libraries and DNA sequencing ...
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All restriction enzymes recognize specif...
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