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Plasmids that govern their own transfer are known as


A) transformable.
B) transmutable.
C) conjugative.
D) transfective.

E) A) and B)
F) A) and C)

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A mutant you made in the laboratory with 5-bromouracil suddenly regains the wild-type phenotype.You discuss this phenomenon with your advisor and colleagues,and they suggest you try transposon mutagenesis to avoid this problem and create stable mutants.Why would mutants created with 5-bromouracil be more likely to regain the wild-type phenotype than mutants created via transposon mutagenesis?

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Because a point mutation involves only a...

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The designations Phe⁻,Leu⁻,and Ser⁺ refer to an organism's


A) plasmid type.
B) genotype.
C) phenotype.
D) mutation type.

E) C) and D)
F) B) and D)

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If a bacterium carrying a plasmid that confers resistance to ampicillin is placed into medium without ampicillin,it may


A) gain resistance to other antibiotics.
B) transfer resistance to other cultures in the laboratory.
C) undergo a reversion mutation.
D) lose the plasmid because there is no selection for ampicillin resistance.

E) B) and C)
F) None of the above

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Lysogeny is essential for the virulence of many pathogenic bacterial strains.

A) True
B) False

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Toxigenicity in Corynebacterium diphtheriae is due to phage conversion.

A) True
B) False

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Compare and contrast generalized transduction with specialized transduction.

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Transduction as a whole is the transfer ...

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You have performed the following mating experiment using Hfr and F⁻ strains of Escherichia coli: Hfr (thr⁺ leu⁺ gal⁺ strˢ) × F⁻ (thr⁻ leu⁻ gal⁻ strʳ) .Which of the following selective media would you use to score recombinant colonies?


A) minimal medium
B) minimal medium + streptomycin
C) minimal medium + threonine
D) minimal medium + streptomycin + threonine

E) A) and D)
F) A) and C)

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Which of the following methods may introduce foreign DNA into a recipient?


A) transformation
B) transduction
C) conjugation
D) transformation,transduction,and conjugation

E) A) and D)
F) B) and D)

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Which process listed below does NOT require homologous recombination to maintain the new genetic material in the recipient cell?


A) conjugation of an F+ plasmid
B) gene transfer through a genetic transfer agent
C) transduction by a dsDNA phage Mu
D) transformation of a linear piece of DNA

E) None of the above
F) B) and C)

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Consider the following experiment.First,large populations of two mutant strains of Escherichia coli are mixed,each requiring a different,single amino acid.After plating them onto a minimal medium,45 colonies grew.Which of the following may explain this result?


A) The colonies may be due to back mutation (reversion) .
B) The colonies may be due to recombination.
C) Either A or B is possible.
D) Neither A nor B is possible.

E) A) and B)
F) C) and D)

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Which of the following features are common to transformation,transduction,and conjugation? (1) unidirectional transfer of genes (2) incomplete gene transfer (3) homologous recombination (4) meiosis occurring in the recipient


A) 1,2,3
B) 1,2
C) 3,4
D) 1,2,4

E) None of the above
F) A) and B)

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The evolutionary significance of phage conversion likely stems from an effective alteration of host cells.

A) True
B) False

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The use of transposons to generate mutations is a convenient way to create bacterial mutations in the laboratory.

A) True
B) False

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Homologous recombination has been observed in


A) Archaea.
B) Bacteria.
C) Eukarya.
D) Archaea,Bacteria,and Eukarya.

E) B) and C)
F) A) and D)

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The positions of genes on a genetic map are marked according to


A) degrees.
B) nanometers.
C) radians.
D) minutes.

E) C) and D)
F) A) and C)

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How might mutations in the How might mutations in the   sequence,whose enzyme is involved in DNA proofreading,be beneficial for a microbe in a highly competitive and dynamic community? sequence,whose enzyme is involved in DNA proofreading,be beneficial for a microbe in a highly competitive and dynamic community?

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Mutations in blured image would result in an increas...

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When UV radiation damage occurs,DNA repair occurs only in the absence of template instruction.

A) True
B) False

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A typical mutation rate for a bacterium is in the range of 10⁻⁶ to 10⁻⁹ per kbp.

A) True
B) False

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The enzyme transposase may be coded for by insertion sequences on a


A) chromosome.
B) phage.
C) plasmid.
D) chromosome,phage,or plasmid.

E) A) and B)
F) A) and C)

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